CLOCK CLIA Kit for Human(Homo sapiens) | EIAab.comCLOCK CLIA Kit for Human(Homo sapiens)

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Index > Protein center > CLOCK(Gene name) > Human

CLOCK (Gene name),

Circadian locomoter output cycles protein kaput (Protein name ), CLOCK_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CLOCK(BHLHE8;KIAA0334);

Protein name:

Circadian locomoter output cycles protein kaput(hCLOCK);

Alternative:

Class E basic helix-loop-helix protein 8(bHLHe8);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif.

Function:

ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL (By similarity). Plays a role in DNA damage response (DDR) signaling during the S phase.

Subcellular Location:

Nucleus Chromosome Cytoplasm Nucleus Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus (By similarity). Localizes to sites of DNA damage in a H2AX-independent manner.

Protein Attributes:

Sequence length:

846

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

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600:

650:

700:

750:

800:

846:

ILSAAFPLQQ | STFPQSHHQQ | HQSQQQQQLS | RHRTDSLPDP | SKVQPQ

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

O15516

KEGG:

hsa:9575

UniGene:

Hs.436975

MIM:

601851

STRING:

9606.ENSP00000308741

String:

O15516

Pfam:

PF00010

Uniprot:

O15516

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ELISA Kit for Human hCLOCK

Cat.:

E15436m

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MSDS:

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Packing:

96T

ELISA Kit for Human hCLOCK

Cat.:

E15436r

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96T

ELISA Kit for Human hCLOCK

Cat.:

E15436c

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96T

ELISA Kit for Human hCLOCK

Cat.:

E15436h

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96T

CLIA Kit for Human hCLOCK

Cat.:

U15436r

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96T

CLIA Kit for Human hCLOCK

Cat.:

U15436c

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96T

CLIA Kit for Human hCLOCK

Cat.:

U15436h

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96T

CLIA Kit for Human hCLOCK

Cat.:

U15436m

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96T

Polyclonal Antibody for Human hCLOCK

Cat.:

P15436Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human hCLOCK

Cat.:

P15436Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human hCLOCK

Cat.:

P15436Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human hCLOCK

Monoclonal Antibody for Human hCLOCK

Protein for Human hCLOCK

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Molecular cloning and characterization of the human CLOCK gene: expression in the suprachiasmatic nuclei."
Steeves T.D.L. , King D.P. , Zhao Y. , Sangoram A.M. , Du F. , Bowcock A.M. , Moore R.Y. , Takahashi J.S.
Genomics57:189-200(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];TISSUE SPECIFICITY
2.

NHLBI resequencing and genotyping service (RS&G)
Submitted (2006-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T. , Ishikawa K. , Nakajima D. , Ohira M. , Seki N. , Miyajima N. , Tanaka A. , Kotani H. , Nomura N. , Ohara O.
DNA Res.4:141-150(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Placenta.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Lung.
6.

"Molecular cloning of human Clock cDNA 5'-end."
Ikeda M. , Takehara N. , Ebisawa T. , Yamauchi T. , Nomura M.
Submitted (1997-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-349
tissue: Brain.
7.

"Regulation of clock and NPAS2 DNA binding by the redox state of NAD cofactors."
Rutter J. , Reick M. , Wu L.C. , McKnight S.L.
Science293:510-514(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DNA-BINDING;ENZYME REGULATION
8.

"Histone acetyltransferase-dependent chromatin remodeling and the vascular clock."
Curtis A.M. , Seo S.B. , Westgate E.J. , Rudic R.D. , Smyth E.M. , Chakravarti D. , FitzGerald G.A. , McNamara P.
J. Biol. Chem.279:7091-7097(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH KAT2B; CREBBP AND EP300
9.

"Feedback repression is required for mammalian circadian clock function."
Sato T.K. , Yamada R.G. , Ukai H. , Baggs J.E. , Miraglia L.J. , Kobayashi T.J. , Welsh D.K. , Kay S.A. , Ueda H.R. , Hogenesch J.B.
Nat. Genet.38:312-319(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF GLU-116; GLY-332; HIS-360; GLU-367; VAL-601 AND PRO-840
10.

"CLOCK/BMAL1 regulates human nocturnin transcription through binding to the E-box of nocturnin promoter."
Li R. , Yue J. , Zhang Y. , Zhou L. , Hao W. , Yuan J. , Qiang B. , Ding J.M. , Peng X. , Cao J.M.
Mol. Cell. Biochem.317:169-177(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
11.

"The transcriptional repressor ID2 can interact with the canonical clock components CLOCK and BMAL1 and mediate inhibitory effects on mPer1 expression."
Ward S.M. , Fernando S.J. , Hou T.Y. , Duffield G.E.
J. Biol. Chem.285:38987-39000(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ID2
12.

"Biochemical analysis of the canonical model for the mammalian circadian clock."
Ye R. , Selby C.P. , Ozturk N. , Annayev Y. , Sancar A.
J. Biol. Chem.286:25891-25902(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARNTL; CRY1 AND PER2
13.

"Peripheral CLOCK regulates target-tissue glucocorticoid receptor transcriptional activity in a circadian fashion in man."
Charmandari E. , Chrousos G.P. , Lambrou G.I. , Pavlaki A. , Koide H. , Ng S.S. , Kino T.
PLoS ONE6:E25612-E25612(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH NR3C1
14.

"A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling."
Cotta-Ramusino C. , McDonald E.R. III , Hurov K. , Sowa M.E. , Harper J.W. , Elledge S.J.
Science332:1313-1317(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
15.

"Histone lysine demethylase JARID1a activates CLOCK-BMAL1 and influences the circadian clock."
DiTacchio L. , Le H.D. , Vollmers C. , Hatori M. , Witcher M. , Secombe J. , Panda S.
Science333:1881-1885(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH KDM5A
16.

"Diurnal expression of the thrombopoietin gene is regulated by CLOCK."
Tracey C.J. , Pan X. , Catterson J.H. , Harmar A.J. , Hussain M.M. , Hartley P.S.
J. Thromb. Haemost.10:662-669(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
17.

"Mechanism of the circadian clock in physiology."
Richards J. , Gumz M.L.
Am. J. Physiol.304:R1053-R1064(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
18.

"Cyclin-dependent kinase 5 (Cdk5) regulates the function of CLOCK protein by direct phosphorylation."
Kwak Y. , Jeong J. , Lee S. , Park Y.U. , Lee S.A. , Han D.H. , Kim J.H. , Ohshima T. , Mikoshiba K. , Suh Y.H. , Cho S. , Park S.K.
J. Biol. Chem.288:36878-36889(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-451 AND THR-461;MUTAGENESIS OF THR-451 AND THR-461;INTERACTION WITH THE COMPLEX P35/CDK5
19.

"p75 neurotrophin receptor is a clock gene that regulates oscillatory components of circadian and metabolic networks."
Baeza-Raja B. , Eckel-Mahan K. , Zhang L. , Vagena E. , Tsigelny I.F. , Sassone-Corsi P. , Ptacek L.J. , Akassoglou K.
J. Neurosci.33:10221-10234(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
20.

"CLOCK is a substrate of SUMO and sumoylation of CLOCK upregulates the transcriptional activity of estrogen receptor-alpha."
Li S. , Wang M. , Ao X. , Chang A.K. , Yang C. , Zhao F. , Bi H. , Liu Y. , Xiao L. , Wu H.
Oncogene32:4883-4891(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION;SUBCELLULAR LOCATION;INTERACTION WITH ESR1
21.

"Metabolism and the circadian clock converge."
Eckel-Mahan K. , Sassone-Corsi P.
Physiol. Rev.93:107-135(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
22.

"A meeting of two chronobiological systems: circadian proteins Period1 and BMAL1 modulate the human hair cycle clock."
Al-Nuaimi Y. , Hardman J.A. , Biro T. , Haslam I.S. , Philpott M.P. , Toth B.I. , Farjo N. , Farjo B. , Baier G. , Watson R.E. , Grimaldi B. , Kloepper J.E. , Paus R.
J. Invest. Dermatol.134:610-619(2014) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY
23.

"Molecular architecture of the mammalian circadian clock."
Partch C.L. , Green C.B. , Takahashi J.S.
Trends Cell Biol.24:90-99(2014) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW

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